Regulatory

Part:BBa_K1513000:Design

Designed by: Hongwei Duan   Group: iGEM14_BUCT-China   (2014-09-18)

A quorum sensing system which can produce auto-inducer (AHL) itself.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1710
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 987


Design Notes

We hoped to adopt a quorum sensing system to accelerate expression of the reporter genes downstream the biosensor. However，the former quorum sensing part(BBa_J09855) required outer AHL. To fix this , we improved it by adding a luxI gene.

Source

partJ09855、partK081008

References